Recent Publications
Roberts R.A., Sabalos C.M., Leblanc M.L., Martel R.R., Frutiger Y.M., Unger J.M., Botros I.W., Rounseville M.P., Seligmann B.E., Miller T.P., Grogan T.M., Rimsza L.M. (2007) “Quantitative nuclease protection assay in paraffin-embedded tissue replicates prognostic microarray gene expression in diffuse large-B-cell lymphoma”, Lab Invest. 87(10), 979-997.
Kris, R.M., Felder, S., Deyholos, M., Lambert, G.M., Hinton, J., Botros,I., Martel, R., Seligmann, B., Galbraith D.W. (2007) “High-Throughput, High-Sensitivity Analysis of Gene Expression in Arabidopsis”, Plant Physiology, 144, 1256-1266.
Sawada H., Taniguchi K., Takami K. (2006) “Improved toxicogenomic screening for drug-induced phospholipidosis using a multiplexed quantitative gene expression ArrayPlate assay”, Toxicol In Vitro. 20(8):1506-13
Martel, R.R., Rounseville, M.P., Botros, I.W. and Seligmann, B.E. (2004) “Array Formats” pp. 3-22 in “Microarray Technology and Its Applications”, ISBN: 3-540-22931-0, Müller, U.R. and Nicolau, D.V., Eds., Springer-Verlag, Heidelberg.
Martel, R. (2003) “Arrays of Possibilities”, Modern Drug Discovery (Published by The American Chemical Society), November 2003, 47-50.
Palfreyman M.G., Hook D.J., Klimczak L.J., Brockman J.A., Evans D.M., Altar C.A. (2002) “Novel directions in antipsychotic target identification using gene arrays” Curr Drug Targets CNS Neurol Disord. 1(2):227-38.
Martel, R.R., Botros, I.W., Rounseville, M.P., Hinton, J.P., Staples, R.R., Morales, D.A., Farmer, J.B., and Seligmann, B.E. (2002) “Multiplexed Screening Assay for mRNA Combining Nuclease Protection with Luminescent Array Detection”, Assay and Drug Development Technologies, 1(1), 61-71.
Martel, R.R., Rounseville, M.P., Botros, I.W. and Seligmann, B.E. (2002) “Multiplexed Chemiluminescent Assays in ArrayPlates for High-Throughput Measurement of Gene Expression”, Progress in Biomedical Optics and Imaging, 3(18), 35-43.
Martel, R.R., Rounseville, M.P., Botros, I.W., Kris, R., Felder, S. and Seligmann, B.E. (2002) “Multiplexed Molecular Profiling (MMP) Transcription Assay in ArrayPlates for High-Throughput Measurement of Gene Expression” pp. 549-564 in “Gene Cloning and Expression Technologies”, ISBN: 1-881299-20-1, Lu, Q. and Weiner, M., Eds., BioTechniques Press, Eaton Publishing, Westborough, MA.
Employment
High Throughput Genomics, Inc. is a Tucson, Arizona based company, but has job opportunities throughout the United States. We are a growing company, and our list of openings changes continuously. Please check this page periodically for new position postings
High Throughput Genomics is an Equal Opportunity Employer.
Please send cover letters and resumes to employment@htgenomics.com.
Tucson, Arizona
Senior R&D Scientist– HTG is looking for a Ph.D. level scientist with significant product development and project management experience. Experience with Design Controls is strongly preferred.
R&D Scientist — HTG is looking for a Master’s level scientist with product design experience. Experience with Design Controls is strongly preferred.
Laboratory Technician
Description: The successful candidate will be a member of HTG’s manufacturing team. The team prepares, packages and ships assay kits to clients who perform their own gene expression assay in-house. The manufacturing team also performs assay services for clients. In this role, the candidate performs all aspects of HTG’s proprietary quantitative Nuclease Protection Assay? and should have experience following an assay protocol and operating/maintaining automated lab equipment, such as plate washers, pipettors, printers, imagers, shakers, incubators. The team provides technical support for proprietary imager sold to clients who perform assays in-house.
Formal Education: A.S. or B.S. degree
Experience: 2-3 years industry or academic laboratory experience, preferably in a manufacturing setting
Additional skills and abilities: Strong math and computer skills (MS Office), ability to follow an assay protocol, ability to operate and maintain automated laboratory equipment, ability to formulate and QC reagent solutions, ability to communicate with clients via telephone or email, ability to analyze data for transmission to clients, and ability to troubleshoot assay or imager failures.
Motivation: Desire to make a difference in research and medicine.
Upcoming shows
Look for us at the 2008 Merck Technology Symposium, May 13th to 16th in Long Branch, NJ. Find out how the qNPA ArrayPlate will enable your research. Visit us at booth 45.
Trademarks
Capella, Omix, qCustom, qFix, and qNPA are trademarks of High Throughput Genomics, Inc. For questions regarding the usage of the marks, please contact feedback@htgenomics.com .
ArrayPlate Pilot Studies
Seeing is believing!
HTG realizes that choosing a new high throughput gene expression platform is a complicated and expensive investment process. To aid in your decision making process, we are happy to provide cost-effective pilot studies which yield real data in your experimental system.
Included in the pilot study are:
– The design of a qCustom™ ArrayPlate Assay possessing up to 16 genes per well.
– The use of our in house service to run a small proof of concept study of up to 5 plates.
– Basic data analysis services for your results.
Customers who wish to continue to use the ArrayPlate technology in their own facilities may be eligible to credit the cost of the pilot study towards the purchase of one of our instruments.
Contact your local sales representative for more details.
Custom Pilot Studies
Interested in qNPA technology, but our standard pilot study doesn’t fit your research needs? Please contact your local sales representative and we’ll work with you to design the pilot study that will show you how effective the qNPA ArrayPlate approach is.
We also offer instrument demos and trials sizes of our qFix™ ArrayPlates for use in your own lab. Contact us for more details
How do I get started?
I’m interested in the qNPA ArrayPlate, what format does it come in?
HTG currently offers the ArrayPlate in two different formats:
Our qFixed Arrays each contain 16 genes focused on a particular functional area. These assays come in a ready to run, preconfigured format. Only a simple ‘tuning’ step is required prior to running the assay. This tuning step can be performed as part experiment is samples are small and precious.
The qCustom system allows researchers to develop their own custom arrays with the gene content of their choice. These arrays are configured and QC’d by our in-house development team to ensure the assay does not contain cross-reactive array elements.
Either ArrayPlate product can be used in your lab (with our imagers) or run by our skilled team at our facility in Tucson, Arizona.
I want to design a qCustom Array. What does HTG need from me?
To begin the design process for your qCustom array, our scientists will need three items:
- A gene list of up to 16 genes that you want represented on your array.
- A lysate sample representing the ‘uninduced’ or basal state of your gene expression targets
- A lysate represented the expected ‘full induction’ of your specified genes
The lysates are used to tune the qCustom array for maximum data coverage for your specific sample. Our experienced scientists will help you determine the appropriate conditions for creating these lysates. Once the array is tuned, it is ready to be used in either your lab or in our service facility.
I am interested in using a qFixed™ Array. What do I need?
To used our qFixed Arrays in your own lab, you will need to have a high resolution luminescence imager; HTG offers two imagers for this purpose. Other imagers may be suitable, please contact us for more information.
Other equipment that is strongly suggested includes robotic liquid handling systems and plate washers. Our scientists have found that, although the assay can be performed with standard or multi-channel pipettes, the reproducibility of the assay improves significantly when automation is used.
What do I need to do to use HTG’s services?
Most of our service customers prepare their cells or other samples (such as tissue or FFPE preparations) in their own facilities using their own specifications. When these treatments are finished, they add qNPA Lysis Buffer (supplied by HTG) to the wells to kill the cells and release the RNA. These lysates are frozen and shipped to HTG on dry ice for immediate processing. We typically deliver the final data set from plates we receive in less than 10 business days.
How much do HTG’s products and services cost?
Please contact your regional sales representative for pricing information. Due to the nature of their projects, many of our customers receive volume discounts. We want to be able to provide you with an accurate estimate of your project costs.
Our pilot studies are an ideal way to familiarize yourself with the power and benefits of the ArrayPlate Technology. Contact us for more information regarding setting a pilot study up for you.
Is the ArrayPlate™ platform right for my research?
Any researcher who is looking to analyze gene expression in a large number of samples for specific gene targets should benefit from using the qNPA ArrayPlate Platform. The ArrayPlate may be especially useful in your research if you are:
- Using large amounts of multiplexed qRT-PCR
- Performing TaqMan® or similar experiments on multiple genes
- Analyzing many samples with microarrays
The ArrayPlate™ platform is designed to be a high throughput assay performed with robotic liquid handling equipment. It is ideally suited to analyze gene targets identified and validated using lower throughput systems. We have found that most of our customers:
- Have used microarrays to identify their initial genes of interest
- Examine a relatively small number of genes to analyze per follow-up sample
- Intend to examine at least 500 samples per experiment
They have turned to using the ArrayPlate Platform due to its fast and simple protocol, low cost per data point, and highly reliable data. Customers with other research needs may benefit from our ArrayPlate or qNPA technologies as well. Please contact your regional account representative to find out if HTG has a solution for your research goals.
Sample Types used with the qNPA™ ArrayPlate™ Assay
The qNPA™ ArrayPlate™ Assay has been used with a wide variety of sample types, including cultured cell lines, fresh tissue pieces, and FFPE archives. For questions regarding your sample type, please contact us for more information.
Immortalized Cell Lines
Extracted RNA
Primary Cell Lines and Tissues
adrenals lung
bladder lymphocytes
blood muscle
brain ovaries
breast pancreas
colon prostate
ear punches spleen
heart testes
kidney umbilical cord
liver
Plants
Maize
Rice
Whole Organisms
Arabidopsis
Drosophila
Gram negative bacteria
Gram positive bacteria
Look for us at the 2008 Merck Technology Symposium, May 13th to 16th in Long Branch, NJ. Find out how the qNPA ArrayPlate will enable your research. Visit us at booth 45.
