Recent Publications
1. Martel, R., Botros, I., Rounseville, M., et al, (2002). Multiplexed screening assay for mRNA combining nuclease protection with luminescent array detection. Assay and Drug Development Technologies, 1 (1-1):61-71.
2. Martel, R., Rounseville, M., Botros, I., et al, (2002). Multiplexed Chemiluminescence Assays in ArrayPlates for High-Throughput Measurement of Gene Expression, Progress in Bimedical Optics and Imaging, 3:35-43.
3. Martel. R., Rounseville, M., Botros, I., et al, (2002). “Multiplexed Molecular Profiling (MMP) Transcription Assay in ArayPlates for High-Throughput Measurement of Gene Expression.” Gene Cloning and Expression Technologies, Q. Lu and M. Weiner, Eds., Eaton Publishing, Natick.
4. Seligmann, B., (2003). “Mining the Transcriptome Using High-Throughput Multiplexed mRNA Profiling.” PharmacoGenomics, 3:36-43.
5. Martel, R., Staples, R., Hinton, J., et al, (2003). “Array Formats” in “Microarray Technologies and Applications”, Microarray Technologies and Applications, Springer-Verlag, Heidelberg.
6. Sawada, H., Taniguchi, K., Takami, K., et al, (2006). “Improved toxicogenomic screening for drug-induced phospholipidosis using a multiplexed quantitative gene expression ArrayPlate assay”. Toxicology in Vitro, 20:1506-1513.
7. Bakir, F., Kher, S., Pannala, M., et al, (2007). “Discovery and structure–activity relationship studies of indole derivatives as liver X receptor (LXR) agonists” Biorg and Med Chem Letters, 17: 3473-3479.
8. Kris, R., Felder, S., Deyholos, M., et al, (2007). “High-Throughput, High-Sensitivity Analysis of Gene Expression in Arabidopsis” Plant Physiol. 144: 1256-1266.
9. Roberts, R., Sabalos, C., Martel, R., et al, ( 2007). “Quantitative Nuclease Protection Assay in Paraffin-Embedded Tissue Replicates Prognostic Microarray Gene Expression in Diffuse Large-B-Cell Lymphoma” Laboratory Investigation, 87: 979-997.
10. Rimsza, L., LeBlanc, M., Unger, J., et al., (2008). “Gene expression predicts overall survival in paraffin embedded tissues of diffuse large B cell lymphoma treated with R-CHOP” Blood, Online June 10, 2008 doi: 10.1182/blood-2008-02-137372.
11. Altar CA, Hunt RA, Jurata LW, Webster MJ, Derby E, Gallagher P, Lemire A, Brockman J, Laeng P (2008). “Insulin, IGF-1, and muscarinic agonists modulate schizophrenia-associated genes in human neuroblastoma cells.” Biological Psychiatry, 64:1077-1087.
12. Fichorova, R. N., Trifonova, R. T., Doncel, G. F., (2009) “Polyanionic Microbicides Modify
Toll-Like Receptor-Mediated Cervicovaginal Immune Responses.” Antimicrobial Agents and
Chemotherapy, April 2009: 1490-1500.
13. Altar CA, Vawter MP, Ginsberg SD (2009). “Target identification for CNS diseases by transcriptional profiling.” Neuropsychopharmacology, 34:18-54.
14. Pechold S, Stouffer M, et. al., (2009) “Transcriptional analysis of intracytoplasmically stained, FACS-purified cells by high throughput, quantitative nuclease protection.” Nature Biotechnology, 27, 1038-1042.
Employment
High Throughput Genomics, Inc. is a Tucson, Arizona based company, but has job opportunities throughout the United States. We are a growing company, and our list of openings changes continuously. Please check this page periodically for new position postings
High Throughput Genomics is an Equal Opportunity Employer.
Please send cover letters and resumes to employment@htgenomics.com.
Tucson, Arizona
Senior Scientist, R&D
HTG is seeking a Ph.D. level biochemist or molecular/cellular biologist to lead programs within its Research and Development Team. R&D programs are focused on new applications and platforms for HTG’s proprietary quantitative Nuclease Protection Assay (qNPA™) gene expression assay technologies. This individual will work at the bench and have responsibilities for leading programs that result in commercial products and publications describing product performance and applications.
Roles and Responsibilities:
Lead programs resulting in commercial products and publications.
Develop multiplexed and quantitative RNA and DNA assays on low and high density array and other platforms.
Required Experience:
-
• Demonstrated industry success in a research and development laboratory.
- A gene list of up to 46 genes that you want represented on your array.
- A lysate sample representing the ‘uninduced’ or basal state of your gene expression targets
- A lysate represented the expected ‘full induction’ of your specified genes
- Using large amounts of multiplexed qRT-PCR
- Performing TaqMan® or similar experiments on multiple genes
- Analyzing many samples with microarrays
- Have used microarrays to identify their initial genes of interest
- Examine a relatively small number of genes to analyze per follow-up sample
- Intend to examine at least 100 samples per experiment
• Proven independence and leadership skills.
• Successful project management experience.
• In depth, hands-on experience with and knowledge of gene expression assay methods, array-based assaymethods, optical detection and optical probes, protein purification and ELISA assays.
• Experience developing diagnostic assays.
• Experience with product development and design controls required.
• Ability to travel.
• Ability to make effective presentations to scientists, management, clients, and investors
Laboratory Technologist
HTG is looking for a laboratory professional to learn and become an expert in our qNPA™ testing procedures and assist in the laboratory’s implementation of an FDA and ISO regulated Quality System. In addition, the position will be responsible for using previous experience to identify and implement improvements throughout the laboratory.
Roles and Responsibilities:
• Perform qNPA testing following standard procedures utilizing a variety of detection methods.
• Sample preparation, including macro-dissecting formalin-fixed paraffin-embedded (FFPE) tissue, FFPE slide scraping, extracted RNA, and fresh frozen tissue lysis.
• Maintain laboratory logs including equipment maintenance, calibration, and temperature.
• Perform Quality Control testing on formulated and purchased reagents.
• Maintains accurate and updated laboratory notebooks and production records
• Adheres to Good Laboratory Practices (GLPs) and Current Good Manufacturing Practices (cGMPs) and appropriate assay protocols
Desired Experience:
Bachelors Degree in a Life Science and/or Medical Technologist certification
• Minimum 2 years experience in a FDA-regulated environment
• Demonstrated expertise with basic laboratory techniques such as pipetting, handling of nucleic acids and proteins, and documentation.
• Experience with molecular techniques, such as PCR, FISH, and microarrays
• Highly organized and detail oriented
• Experience with FFPE tissue (histological samples) is desirable.
Executive Account Representatives (Various Locations)
Baltimore / Washington D.C.
Houston / Dallas
HTG is seeking highly credible and passionate individuals with advanced degrees (preferred) in one of the life sciences for field positions. The Company is seeking candidates who currently live in the aforementioned major metropolitan regions and who have strong sales experience to develop strategic sales plans, achieve sales, and monitor the marketplace to respond to changes in the competitive market. Candidates must possess excellent organizational, presentation, and communications skills. Successful candidates will be analytical thinkers capable of persuading and building consensus in large organizations and operating remotely and independently.
Role and Responsibilities:
• Develop a territory and account-specific sales plans.
• Execute plan to meet sales quotas.
• Use and maintain CRM.
• Deliver technical/scientific seminars and presentations about HTG products and their capabilities.
• Demonstrate role and relevance of HTG’s product portfolio and provide consultation to customers regarding the portfolio.
• Demonstrate command of scientific knowledge to engage in collaborative sale.
• Develop and maintain strong customer connections at various levels an organization.
• Minimum 50% field travel.
Mandatory Qualifications:
• Advanced life science degree (Ph.D. or Master’s in Biochemistry or Molecular Biology preferred). Must be able to understand and explain molecular biology based technology and products.
• 5+ years commercial experience, preferably with core experience in life science or biotech companies. Strong preference for experience with research and laboratory products.
• Preferred candidates will have previously demonstrated success with complex sales and within multi-layered environments.
• Candidates must be a motivated self-starter with proactive prospecting skills, be highly adaptable and independent and have a strong desire to win business and establish long-term customer relationships.
Trademarks
Capella, SuperCapella, Omix, OMIX HD, qCustom, qFix, qSelect, and qNPA are trademarks of High Throughput Genomics, Inc. For questions regarding the usage of the marks, please contact feedback@htgenomics.com .
ArrayPlate Pilot Studies
ArrayPlate Pilot Studies
HTG realizes that choosing a new high throughput gene expression platform is a complicated and expensive investment process. To aid in your decision making process, we are happy to provide cost-effective pilot studies which yield real data in your experimental system.
Included in the pilot study are:
– The design of an up to 45 gene qCustom™ ArrayPlate Assay.
– The use of our in house service to run a small proof of concept study of up to five 96 well plates.
– Basic data analysis services for your results.
Contact us at support@htgenomics.com for more details.
Custom Pilot Studies
Interested in qNPA technology, but our standard pilot study doesn’t fit your research needs? Please contact your local sales representative and we’ll work with you to design the pilot study that will show you how effective the qNPA ArrayPlate approach is.
We also offer instrument demos and trials sizes of our qFix™ ArrayPlates for use in your own lab. Contact us for more details.
Brochures
Academic Research -
High Throughput Gene Expression Analysis
Drug Discovery -
Multiplexed Biomarker-Driven Drug Discovery
How do I get started?
I’m interested in the qNPA ArrayPlate, what format does it come in?
HTG currently offers the ArrayPlate in two different formats:
Our qFixed Arrays each contain 16 genes focused on a particular functional area. These assays come in a ready to run, preconfigured format. Only a simple ‘tuning’ step is required prior to running the assay. This tuning step can be performed as part experiment is samples are small and precious.
The qCustom system allows researchers to develop their own custom arrays with the gene content of their choice. These arrays are configured and QC’d by our in-house development team to ensure the assay does not contain cross-reactive array elements.
Either ArrayPlate product can be used in your lab (with our imagers) or run by our skilled team at our facility in Tucson, Arizona.
I want to design a qCustom Array. What does HTG need from me?
To begin the design process for your qCustom array, our scientists will need three items:
The lysates are used to tune the qCustom array for maximum data coverage for your specific sample. Our experienced scientists will help you determine the appropriate conditions for creating these lysates. Once the array is tuned, it is ready to be used in either your lab or in our service facility.
I am interested in using a qFix™ ArrayPlate. What do I need?
To used our qFixed Arrays in your own lab, you will need to have a high resolution luminescence imager; HTG offers two imagers for this purpose. Other imagers may be suitable, please contact us for more information.
Other equipment that is strongly suggested includes robotic liquid handling systems and plate washers. Our scientists have found that, although the assay can be performed with standard or multi-channel pipettes, the reproducibility of the assay improves significantly when automation is used.
What do I need to do to use HTG’s services?
Most of our service customers prepare their cells or other samples (such as tissue or FFPE preparations) in their own facilities using their own specifications. When these treatments are finished, they add qNPA Lysis Buffer (supplied by HTG) to the wells to kill the cells and release the RNA. These lysates are frozen and shipped to HTG on dry ice for immediate processing. We typically deliver the final data set from plates we receive in less than 10 business days.
How much do HTG’s products and services cost?
Please contact your regional sales representative for pricing information. Due to the nature of their projects, many of our customers receive volume discounts. We want to be able to provide you with an accurate estimate of your project costs.
Our pilot studies are an ideal way to familiarize yourself with the power and benefits of the ArrayPlate Technology. Contact us for more information regarding setting a pilot study up for you.
Is the ArrayPlate platform right for my research?
Any researcher who is looking to analyze gene expression in a large number of samples for specific gene targets should benefit from using the qNPA™ ArrayPlate Platform. The ArrayPlate may be especially useful in your research if you are:
The ArrayPlate platform is designed to be a high throughput assay performed with robotic liquid handling equipment. It is ideally suited to analyze gene targets identified and validated using lower throughput systems. We have found that most of our customers:
They have turned to using the ArrayPlate Platform due to its fast and simple protocol, low cost per data point, and highly reliable data. Customers with other research needs may benefit from our ArrayPlate or qNPA technologies as well. Please contact your regional account representative to find out if HTG has a solution for your research goals.
Sample Types used with the qNPA™ ArrayPlate™ Assay
The qNPA™ ArrayPlate™ Assay has been used with a wide variety of sample types, including cultured cell lines, fresh tissue pieces, and FFPE archives. For questions regarding your sample type, please contact us for more information.
Immortalized Cell Lines
Extracted RNA
FFPE and H & E Stained Slides
Primary Cell Lines and Tissues
adrenals lung
bladder lymphocytes
blood muscle
brain ovaries
breast pancreas
colon prostate
ear punches spleen
heart testes
kidney umbilical cord
liver
Plants
Maize
Rice
Whole Organisms
Arabidopsis
Drosophila
Gram negative bacteria
Gram positive bacteria
Vienna, Austria
San Jose, CA
