How do I get started?
I’m interested in the qNPA ArrayPlate, what format does it come in?
HTG currently offers the ArrayPlate in two different formats:
Our qFixed Arrays each contain 16 genes focused on a particular functional area. These assays come in a ready to run, preconfigured format. Only a simple ‘tuning’ step is required prior to running the assay. This tuning step can be performed as part experiment is samples are small and precious.
The qCustom system allows researchers to develop their own custom arrays with the gene content of their choice. These arrays are configured and QC’d by our in-house development team to ensure the assay does not contain cross-reactive array elements.
Either ArrayPlate product can be used in your lab (with our imagers) or run by our skilled team at our facility in Tucson, Arizona.
I want to design a qCustom Array. What does HTG need from me?
To begin the design process for your qCustom array, our scientists will need three items:
- A gene list of up to 16 genes that you want represented on your array.
- A lysate sample representing the ‘uninduced’ or basal state of your gene expression targets
- A lysate represented the expected ‘full induction’ of your specified genes
The lysates are used to tune the qCustom array for maximum data coverage for your specific sample. Our experienced scientists will help you determine the appropriate conditions for creating these lysates. Once the array is tuned, it is ready to be used in either your lab or in our service facility.
I am interested in using a qFixed™ Array. What do I need?
To used our qFixed Arrays in your own lab, you will need to have a high resolution luminescence imager; HTG offers two imagers for this purpose. Other imagers may be suitable, please contact us for more information.
Other equipment that is strongly suggested includes robotic liquid handling systems and plate washers. Our scientists have found that, although the assay can be performed with standard or multi-channel pipettes, the reproducibility of the assay improves significantly when automation is used.
What do I need to do to use HTG’s services?
Most of our service customers prepare their cells or other samples (such as tissue or FFPE preparations) in their own facilities using their own specifications. When these treatments are finished, they add qNPA Lysis Buffer (supplied by HTG) to the wells to kill the cells and release the RNA. These lysates are frozen and shipped to HTG on dry ice for immediate processing. We typically deliver the final data set from plates we receive in less than 10 business days.
How much do HTG’s products and services cost?
Please contact your regional sales representative for pricing information. Due to the nature of their projects, many of our customers receive volume discounts. We want to be able to provide you with an accurate estimate of your project costs.
Our pilot studies are an ideal way to familiarize yourself with the power and benefits of the ArrayPlate Technology. Contact us for more information regarding setting a pilot study up for you.
