qNPA Technology for measuring miRNA and short RNAs
Detecting miRNA with qNPA Technology is achieved through minor modifications of our qNPA protocol for mRNA. These modified conditions are necessitated by the short sequences provided by the RNA targets, and include direct biotinylation of the qNPA Nuclease Protection Probes and a lower hybridization temperature due to the lower melting temperature of the short RNA:DNA hybrids.
The qNPA process has the proven flexibility to perform either a broad view scan of all known miRNAs from an organism or a smaller, focused panel to look for specific signatures. miRNA measurement can be performed from any sample type, including FFPE, blood plasma, and fresh tissues with the same simple protocol.
For information regarding qNPA products for miRNA follow these links:
qNPA miRNA Microarrays for whole-transcriptome coverage of human, rat and mouse.
qSelect miRNA Archive for up to 47 analyte miRNA signatures on the qNPA ArrayPlate.
qCustom miRNA ArrayPlate for the measurement of mRNA and miRNA in the same well, tRNAs, non-mammalian miRNAs, siRNAs, and other small RNA molecules.
Key miRNA with qNPA Features:
• No RNA extraction – Removes a tedious, highly variable step in the miRNA detection process. Also allows smaller amounts of sample to be used.
• Works with any sample type – Difficult sample types such as FFPE, blood plasma, and intact tissues can all be assayed with similar ease.
• Measure mRNA and miRNAs in the same sample well – Avoid data analysis problems; use validated mRNA housekeeping genes to normalize miRNA experiments.
• Fast, simple workflow – No complicated molecular additions or modifications, simply uses high-stringency hybridization and S1 nuclease digestion.
