Measuring miRNA in the qNPA™ ArrayPlate.
The measurement of miRNA on the qNPA ArrayPlate uses a modified version of the ArrayPlate detection scheme. miRNA detection on the ArrayPlate features:
- Whole-plate CV’s of biological replicates are typically ~10%
- Detection of as few as 15,000 miRNA molecules
- mRNA and miRNA measurement simultaneously in the same sample well
For additional information about miRNA Detection on the qNPA ArrayPlate, consult our miRNA FAQ.
miRNA ArrayPlate Detection
The ArrayPlate spots are programmed using the appropriate gene-specific Programming Linkers which provide a specific capture sequence for the targeted miRNA.The array spots can be programmed to detect both mRNA and micro RNA in the same well, simplifying the studies involving both types of RNAs.
The qNPA protection oligos are added to the ArrayPlate wells and allowed to hybridize (click here for a demonstration of the qNPA™ process). The short, biotinylated , 21 to 23 nucleotide miRNA protection oligos quantitatively hybridize to the programmed array spots.
The array-bound biotinylated miRNA qNPA protection oligos are labeled with an Avidin – horseradish peroxidase (HRP) conjugate. Addition of the chemiluminescent substrate produces light which is then quantitatively measured. For a description of the data collection process, click here.
